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Image Search Results
Journal: EJNMMI Research
Article Title: A comparison of 111 In- or 64 Cu-DOTA-trastuzumab Fab fragments for imaging subcutaneous HER2-positive tumor xenografts in athymic mice using microSPECT/CT or microPET/CT
doi: 10.1186/2191-219X-1-15
Figure Lengend Snippet: SDS-PAGE and size-exclusion HPLC . (a) SDS-PAGE analysis on a 4% to 20% Tris HCl gradient mini-gel stained with Coomassie Brilliant Blue of trastuzumab (lane 1), trastuzumab Fab (lane 2), and DOTA-trastuzumab Fab (lane 3) under non-reducing conditions or these same proteins under reducing conditions (lanes 4 to 6, respectively). Molecular weight markers are shown (lane MW). The positions of 150, 50, and 25 kDa markers are indicated. Size-exclusion HPLC analyses of ( b ) 111 In-DOTA-trastuzumab Fab (top panel) and ( c ) 64 Cu-DOTA-trastuzumab Fab (bottom panel) with detection of absorbance at 280 nm or radioactivity.
Article Snippet: Fab purity was assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on a 4% to 20%
Techniques: SDS Page, Staining, Molecular Weight, Radioactivity